My original tumblr post was here. This animation was researched by Bacchus at ErosBlog as part of the “Δ commission.” The research was originally published at Hedonix as “Δ 055 – Brain-In-A-Jar Villain.” Here is what Bacchus found.

The source Tumblr post in this animation’s current Tumblr chain of provenance includes the tags “#bare knuckle 3 #sega #mega drive”, suggesting that the animation is from scenes included in the 1994 Sega console game Streets of Rage 3 (titled Bare Knuckle III in Japan) for the Sega Genesis (released in Japan in 1988 as the Mega Drive).

A video of the final scenes of the game (in which a player first defeats the final sub-boss before entering the room shown in this animation) may be viewed on Youtube. Before confronting the ultimate boss monster, the player has a short text conversation with the brain in the jar, who is called “Mr. X.” The player asks Mr. X “Do you expect to run the city from a glass phial?” Mr. X says “Yes, traitor, let me show you how!” and then the final toughest robot combat minion appears. In the fight the brain’s jar is broken and Dr. X then says “I’m dying. Please help me.”